rabbit anti pstat3  (Cell Signaling Technology Inc)

Journal: Frontiers in Cellular Neuroscience

Article Title: Adipose-derived stem cell-conditioned medium mitigates ischemia-induced neuronal injury via the JAK1/STAT3 signaling pathway

doi: 10.3389/fncel.2026.1744887

Figure Lengend Snippet: ADSC-CM promotes neurological functional recovery in tMCAO rats through the JAK1-STAT3 signaling pathway. (A) Western blot bands of JAK1, pJAK1, STAT3, pSTAT3, and GAPDH for each group. (B) Comparison of the relative ratio of pJAK1 to JAK1 across experimental groups ( n = 4). (C) Comparison of the relative ratio of pSTAT3 to STAT3 across experimental groups ( n = 4). (D) The line graph displays the trend in mNSS scores over time (days 1, 3, and 7) after tMCAO in each group. (E) The bar graph compares mNSS scores on days 1, 3, and 7 after tMCAO across experimental groups ( n = 16). Data are expressed as means ± SEM. The differences revealed by western blot and in mNSS scores were assessed by one-way and two-way ANOVA, respectively, both followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA) in tris-buffered saline containing 0.1% Tween 20 (TBS-T) at room temperature for 2 h, followed by overnight incubation at 4 °C with primary antibodies: mouse anti-JAK1 antibody (1:1000, 66466-1-Ig, Proteintech, Chicago, IL, USA), rabbit anti-pJAK1 antibody (1:1000, AF2012, Affinity, Jiangsu, China), rabbit anti-STAT3 antibody (1:1000, 10253-2-AP, Proteintech), rabbit anti-pSTAT3 antibody (1:1000, AF3293, Affinity), and mouse anti-GAPDH antibody (1:1000, AF0006, Beyotime).

Techniques: Functional Assay, Western Blot, Comparison

Journal: Frontiers in Cellular Neuroscience

Article Title: Adipose-derived stem cell-conditioned medium mitigates ischemia-induced neuronal injury via the JAK1/STAT3 signaling pathway

doi: 10.3389/fncel.2026.1744887

Figure Lengend Snippet: ADSC-CM promotes neurite outgrowth in OGD-injured neurons through the JAK1-STAT3 signaling pathway. (A) On day 5 of cultivation, neuronal neurites extended and formed an extensive neural network. Immunofluorescence staining showed that the neurons were positive for the neuronal-specific marker Tuj1. After OGD injury, neuronal neurites were damaged, partially interrupted, and disappeared. (B) Western blot bands of JAK1, pJAK1, STAT3, pSTAT3, and GAPDH for each group. (C) Comparison of the relative ratio of pJAK1 to JAK1 across experimental groups ( n = 4). (D) Comparison of the relative ratio of pSTAT3 to STAT3 across experimental groups ( n = 4). (E) Immunofluorescence images of neurons from each group, showing the effects of ADSC-CM and GLPG0634 on neurite outgrowth. Neurons were stained with the Tuj1 antibody (red) to label the neuronal cytoskeleton, and nuclei were counterstained with DAPI (blue). (F) Diagram illustrating how to measure the length of the longest neurite and the number of primary neurites in neurons. The green line shows the trajectory of the longest neurite, and white arrows point to primary neurites. (G,H) Comparison of the length of the longest neurite and the number of primary neurites in neurons across experimental groups ( n = 4). Data are expressed as means ± SEM. The difference between the groups was assessed using a one-way ANOVA followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bars: 100 μm (A) , 20 μm (E,F) .

Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA) in tris-buffered saline containing 0.1% Tween 20 (TBS-T) at room temperature for 2 h, followed by overnight incubation at 4 °C with primary antibodies: mouse anti-JAK1 antibody (1:1000, 66466-1-Ig, Proteintech, Chicago, IL, USA), rabbit anti-pJAK1 antibody (1:1000, AF2012, Affinity, Jiangsu, China), rabbit anti-STAT3 antibody (1:1000, 10253-2-AP, Proteintech), rabbit anti-pSTAT3 antibody (1:1000, AF3293, Affinity), and mouse anti-GAPDH antibody (1:1000, AF0006, Beyotime).

Techniques: Immunofluorescence, Staining, Marker, Western Blot, Comparison