rabbit anti pstat3 (Cell Signaling Technology Inc)
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Rabbit Anti Pstat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 6117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+pstat3/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pm41912889-71-31-34
Average 98 stars, based on 6117 article reviews
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Incubation:Article Title: TonEBP as a key regulator of hypothalamic leptin signaling and resistance. Article Snippet: Coronal sections (50 μm) containing the ARC of the hypothalamus were prepared using a vibratome (MW-1000, Meiwa Shoji Co., Japan). .. Sections were permeabilized with 0.4% Triton X100 in PB for 30 min, washed, and incubated overnight at 4°C with the following primary antibodies: rabbit anti-TonEBP (1:1000; Thermo Scientific, Catalogue No. PA1-023), Article Title: Identification of an epigenetically and phenotypically distinct peritumoral glioblastoma cell population linked to inferior patient outcome Article Snippet: Equal amounts of protein samples were resolved on 4–12% Bis-Tris polyacrylamide gradient gels (NuPAGE, Thermo Scientific) and transferred onto nitrocellulose membranes (Novex, Thermo Scientific). .. Membranes were blocked with EveryBlot Blocking Buffer (BioRad, 12010020) and incubated overnight at 4°C with the following primary antibodies: Article Title: Neuroprotective Effects of Time-Restricted Feeding Combined With Different Protein Sources in MPTP-Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism. Article Snippet: The membrane was then blocked with 5% (w/v) skim milk (36120ES76, Yeasen Biotech, China) in Tris-buffered saline containing 0.1% Tween-20 (P1379, Sigma-Aldrich, USA) (TBST) for 2 h at room temperature. .. Each membranes were separately incubated overnight at 4◦C with specific antibodies: rabbit anti-TH (1:1000, MAB152, Millipore, USA), rabbit antiMAO-B (1:1000, 12602-1-AP, Proteintech, China), rabbit anti-ZO-1 (1:1000, 21773-1-AP, Proteintech, China), rabbit anti-Occludin (1:1000, 27260-1-AP, Proteintech, China), rabbit anti-Claudin-1 (1:200, TA0127, Abmart, China), rabbit anti-p-NF- κB (Ser536, 1:1000, 3033, Cell Signaling Technology, USA), rabbit anti-NF- κB (1:1000, 8242, Cell Signaling Technology, USA), Article Title: Sex-specific hypothalamic axis disruption in the rNLS8 mouse model of amyotrophic lateral sclerosis (ALS) Article Snippet: RIPA buffer (Sigma Aldrich) containing a cocktail of protease inhibitors (Roche) as described previously [ ] was used to extract proteins from spinal cord. .. 20 μg of denatured protein samples from each group were electrophoresed into Bolt® Bis–Tris Plus gels (Invitrogen), transferred to PVDF membranes (BioRad) and incubated with primary antibodies [rabbit anti-ObRb (1:500; Abcam), rabbit anti-leptin (1:400, Invitro), mouse anti- STAT3 (1:500; Santa Cruz), Blocking Assay:Article Title: Identification of an epigenetically and phenotypically distinct peritumoral glioblastoma cell population linked to inferior patient outcome Article Snippet: Equal amounts of protein samples were resolved on 4–12% Bis-Tris polyacrylamide gradient gels (NuPAGE, Thermo Scientific) and transferred onto nitrocellulose membranes (Novex, Thermo Scientific). .. Membranes were blocked with EveryBlot Blocking Buffer (BioRad, 12010020) and incubated overnight at 4°C with the following primary antibodies: Staining:Article Title: A uniquely leptin sensitive hypothalamic neuron population limits hyperphagia and weight gain in diet-induced obesity Article Snippet: .. Phosphorylated STAT3 antibody staining was performed in KPBS with 1% NGS and 0.3% Triton X-100 with Next-Generation Sequencing:Article Title: A uniquely leptin sensitive hypothalamic neuron population limits hyperphagia and weight gain in diet-induced obesity Article Snippet: .. Phosphorylated STAT3 antibody staining was performed in KPBS with 1% NGS and 0.3% Triton X-100 with other:Article Title: TGFβ signaling in cancer-associated fibroblasts drives a hepatic gp130-dependent pro-metastatic inflammatory program in colorectal cancer Article Snippet: |
