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rabbit anti pstat3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti pstat3
    Rabbit Anti Pstat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 6117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+pstat3/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pm41912889-71-31-34
    Average 98 stars, based on 6117 article reviews
    rabbit anti pstat3 - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: TonEBP as a key regulator of hypothalamic leptin signaling and resistance.
    Article Snippet: Coronal sections (50 μm) containing the ARC of the hypothalamus were prepared using a vibratome (MW-1000, Meiwa Shoji Co., Japan). .. Sections were permeabilized with 0.4% Triton X100 in PB for 30 min, washed, and incubated overnight at 4°C with the following primary antibodies: rabbit anti-TonEBP (1:1000; Thermo Scientific, Catalogue No. PA1-023), rabbit anti-pSTAT3 (1:2000; Cell Signaling Technology, Catalogue No. 9145), rabbit anti-cFos (1:1000; Santa Cruz, .. AR TIC LE IN PR ES S ARTICLE IN PRESS California, CA, Catalogue No. sc-52) and sheep anti-α-MSH (1:5000; MilliporeSigma, St. Louis, MO, Catalogue No. AB5087).

    Article Title: Identification of an epigenetically and phenotypically distinct peritumoral glioblastoma cell population linked to inferior patient outcome
    Article Snippet: Equal amounts of protein samples were resolved on 4–12% Bis-Tris polyacrylamide gradient gels (NuPAGE, Thermo Scientific) and transferred onto nitrocellulose membranes (Novex, Thermo Scientific). .. Membranes were blocked with EveryBlot Blocking Buffer (BioRad, 12010020) and incubated overnight at 4°C with the following primary antibodies: rabbit anti-pSTAT3 (#9145, CST), mouse anti-STAT3 (#9139, CST), rabbit anti-pERK1/2 (#9101, CST), rabbit anti-ERK1/2 (#9102, CST), rabbit anti-pAKT(#4060, CST), rabbit anti-AKT(#4691, CST) and rabbit anti-GAPDH (#5174, CST). .. Following primary antibody incubation, membranes were incubated with HRP-conjugated secondary antibodies (anti-mouse, GE Healthcare, #NXA931; anti-rabbit, GE Healthcare, #NA9340) and developed using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific, 34095).

    Article Title: Neuroprotective Effects of Time-Restricted Feeding Combined With Different Protein Sources in MPTP-Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism.
    Article Snippet: The membrane was then blocked with 5% (w/v) skim milk (36120ES76, Yeasen Biotech, China) in Tris-buffered saline containing 0.1% Tween-20 (P1379, Sigma-Aldrich, USA) (TBST) for 2 h at room temperature. .. Each membranes were separately incubated overnight at 4◦C with specific antibodies: rabbit anti-TH (1:1000, MAB152, Millipore, USA), rabbit antiMAO-B (1:1000, 12602-1-AP, Proteintech, China), rabbit anti-ZO-1 (1:1000, 21773-1-AP, Proteintech, China), rabbit anti-Occludin (1:1000, 27260-1-AP, Proteintech, China), rabbit anti-Claudin-1 (1:200, TA0127, Abmart, China), rabbit anti-p-NF- κB (Ser536, 1:1000, 3033, Cell Signaling Technology, USA), rabbit anti-NF- κB (1:1000, 8242, Cell Signaling Technology, USA), rabbit anti-pSTAT3 (Tyr705, 1:1000, 9145, Cell Signaling Technology, USA), rabbit anti-STAT3 (1:1000, 4904, Cell Signaling Technology, USA), rabbit anti-p-Akt (Ser473, 1:1000, 4060, Cell Signaling Technology, USA), rabbit anti-Akt (1:1000, 4691, Cell Signaling Technology, USA) and rabbit anti-GAPDH (1:8000, 10494-1-AP, Proteintech, China). .. After washing 3 times with TBST for 10 min each at room temperature, goat anti-rabbit IgG (1:8000, BA1054, Boster, China) conjugated to horseradish peroxidase were used as secondary antibody and incubated for 2 h at room temperature.

    Article Title: Sex-specific hypothalamic axis disruption in the rNLS8 mouse model of amyotrophic lateral sclerosis (ALS)
    Article Snippet: RIPA buffer (Sigma Aldrich) containing a cocktail of protease inhibitors (Roche) as described previously [ ] was used to extract proteins from spinal cord. .. 20 μg of denatured protein samples from each group were electrophoresed into Bolt® Bis–Tris Plus gels (Invitrogen), transferred to PVDF membranes (BioRad) and incubated with primary antibodies [rabbit anti-ObRb (1:500; Abcam), rabbit anti-leptin (1:400, Invitro), mouse anti- STAT3 (1:500; Santa Cruz), rabbit anti-pSTAT3 (1:1000; Cell Signalling), mouse anti-Akt (1:500; Santa Cruz), rabbit anti-pAkt (1:500; Cell Signaling)] overnight. .. A corresponding anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (Vector Laboratories) at dilution of 1:5000 was used as described previously [ ].

    Blocking Assay:

    Article Title: Identification of an epigenetically and phenotypically distinct peritumoral glioblastoma cell population linked to inferior patient outcome
    Article Snippet: Equal amounts of protein samples were resolved on 4–12% Bis-Tris polyacrylamide gradient gels (NuPAGE, Thermo Scientific) and transferred onto nitrocellulose membranes (Novex, Thermo Scientific). .. Membranes were blocked with EveryBlot Blocking Buffer (BioRad, 12010020) and incubated overnight at 4°C with the following primary antibodies: rabbit anti-pSTAT3 (#9145, CST), mouse anti-STAT3 (#9139, CST), rabbit anti-pERK1/2 (#9101, CST), rabbit anti-ERK1/2 (#9102, CST), rabbit anti-pAKT(#4060, CST), rabbit anti-AKT(#4691, CST) and rabbit anti-GAPDH (#5174, CST). .. Following primary antibody incubation, membranes were incubated with HRP-conjugated secondary antibodies (anti-mouse, GE Healthcare, #NXA931; anti-rabbit, GE Healthcare, #NA9340) and developed using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific, 34095).

    Staining:

    Article Title: A uniquely leptin sensitive hypothalamic neuron population limits hyperphagia and weight gain in diet-induced obesity
    Article Snippet: .. Phosphorylated STAT3 antibody staining was performed in KPBS with 1% NGS and 0.3% Triton X-100 with rabbit anti-pSTAT3 (Cell Signaling Technology, #9145; 1:200) overnight at 4°C. ..

    Next-Generation Sequencing:

    Article Title: A uniquely leptin sensitive hypothalamic neuron population limits hyperphagia and weight gain in diet-induced obesity
    Article Snippet: .. Phosphorylated STAT3 antibody staining was performed in KPBS with 1% NGS and 0.3% Triton X-100 with rabbit anti-pSTAT3 (Cell Signaling Technology, #9145; 1:200) overnight at 4°C. ..

    other:

    Article Title: TGFβ signaling in cancer-associated fibroblasts drives a hepatic gp130-dependent pro-metastatic inflammatory program in colorectal cancer
    Article Snippet: Rabbit anti-pSTAT3 (clone D3A7) , Cell Signaling Technology , Cat#9145; RRID: AB_2491009.



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    ADSC-CM promotes neurological functional recovery in tMCAO rats through the JAK1-STAT3 signaling pathway. (A) Western blot bands of JAK1, pJAK1, STAT3, <t>pSTAT3,</t> and GAPDH for each group. (B) Comparison of the relative ratio of pJAK1 to JAK1 across experimental groups ( n = 4). (C) Comparison of the relative ratio of pSTAT3 to STAT3 across experimental groups ( n = 4). (D) The line graph displays the trend in mNSS scores over time (days 1, 3, and 7) after tMCAO in each group. (E) The bar graph compares mNSS scores on days 1, 3, and 7 after tMCAO across experimental groups ( n = 16). Data are expressed as means ± SEM. The differences revealed by western blot and in mNSS scores were assessed by one-way and two-way ANOVA, respectively, both followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Image Search Results


    ADSC-CM promotes neurological functional recovery in tMCAO rats through the JAK1-STAT3 signaling pathway. (A) Western blot bands of JAK1, pJAK1, STAT3, pSTAT3, and GAPDH for each group. (B) Comparison of the relative ratio of pJAK1 to JAK1 across experimental groups ( n = 4). (C) Comparison of the relative ratio of pSTAT3 to STAT3 across experimental groups ( n = 4). (D) The line graph displays the trend in mNSS scores over time (days 1, 3, and 7) after tMCAO in each group. (E) The bar graph compares mNSS scores on days 1, 3, and 7 after tMCAO across experimental groups ( n = 16). Data are expressed as means ± SEM. The differences revealed by western blot and in mNSS scores were assessed by one-way and two-way ANOVA, respectively, both followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Adipose-derived stem cell-conditioned medium mitigates ischemia-induced neuronal injury via the JAK1/STAT3 signaling pathway

    doi: 10.3389/fncel.2026.1744887

    Figure Lengend Snippet: ADSC-CM promotes neurological functional recovery in tMCAO rats through the JAK1-STAT3 signaling pathway. (A) Western blot bands of JAK1, pJAK1, STAT3, pSTAT3, and GAPDH for each group. (B) Comparison of the relative ratio of pJAK1 to JAK1 across experimental groups ( n = 4). (C) Comparison of the relative ratio of pSTAT3 to STAT3 across experimental groups ( n = 4). (D) The line graph displays the trend in mNSS scores over time (days 1, 3, and 7) after tMCAO in each group. (E) The bar graph compares mNSS scores on days 1, 3, and 7 after tMCAO across experimental groups ( n = 16). Data are expressed as means ± SEM. The differences revealed by western blot and in mNSS scores were assessed by one-way and two-way ANOVA, respectively, both followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA) in tris-buffered saline containing 0.1% Tween 20 (TBS-T) at room temperature for 2 h, followed by overnight incubation at 4 °C with primary antibodies: mouse anti-JAK1 antibody (1:1000, 66466-1-Ig, Proteintech, Chicago, IL, USA), rabbit anti-pJAK1 antibody (1:1000, AF2012, Affinity, Jiangsu, China), rabbit anti-STAT3 antibody (1:1000, 10253-2-AP, Proteintech), rabbit anti-pSTAT3 antibody (1:1000, AF3293, Affinity), and mouse anti-GAPDH antibody (1:1000, AF0006, Beyotime).

    Techniques: Functional Assay, Western Blot, Comparison

    ADSC-CM promotes neurite outgrowth in OGD-injured neurons through the JAK1-STAT3 signaling pathway. (A) On day 5 of cultivation, neuronal neurites extended and formed an extensive neural network. Immunofluorescence staining showed that the neurons were positive for the neuronal-specific marker Tuj1. After OGD injury, neuronal neurites were damaged, partially interrupted, and disappeared. (B) Western blot bands of JAK1, pJAK1, STAT3, pSTAT3, and GAPDH for each group. (C) Comparison of the relative ratio of pJAK1 to JAK1 across experimental groups ( n = 4). (D) Comparison of the relative ratio of pSTAT3 to STAT3 across experimental groups ( n = 4). (E) Immunofluorescence images of neurons from each group, showing the effects of ADSC-CM and GLPG0634 on neurite outgrowth. Neurons were stained with the Tuj1 antibody (red) to label the neuronal cytoskeleton, and nuclei were counterstained with DAPI (blue). (F) Diagram illustrating how to measure the length of the longest neurite and the number of primary neurites in neurons. The green line shows the trajectory of the longest neurite, and white arrows point to primary neurites. (G,H) Comparison of the length of the longest neurite and the number of primary neurites in neurons across experimental groups ( n = 4). Data are expressed as means ± SEM. The difference between the groups was assessed using a one-way ANOVA followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bars: 100 μm (A) , 20 μm (E,F) .

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Adipose-derived stem cell-conditioned medium mitigates ischemia-induced neuronal injury via the JAK1/STAT3 signaling pathway

    doi: 10.3389/fncel.2026.1744887

    Figure Lengend Snippet: ADSC-CM promotes neurite outgrowth in OGD-injured neurons through the JAK1-STAT3 signaling pathway. (A) On day 5 of cultivation, neuronal neurites extended and formed an extensive neural network. Immunofluorescence staining showed that the neurons were positive for the neuronal-specific marker Tuj1. After OGD injury, neuronal neurites were damaged, partially interrupted, and disappeared. (B) Western blot bands of JAK1, pJAK1, STAT3, pSTAT3, and GAPDH for each group. (C) Comparison of the relative ratio of pJAK1 to JAK1 across experimental groups ( n = 4). (D) Comparison of the relative ratio of pSTAT3 to STAT3 across experimental groups ( n = 4). (E) Immunofluorescence images of neurons from each group, showing the effects of ADSC-CM and GLPG0634 on neurite outgrowth. Neurons were stained with the Tuj1 antibody (red) to label the neuronal cytoskeleton, and nuclei were counterstained with DAPI (blue). (F) Diagram illustrating how to measure the length of the longest neurite and the number of primary neurites in neurons. The green line shows the trajectory of the longest neurite, and white arrows point to primary neurites. (G,H) Comparison of the length of the longest neurite and the number of primary neurites in neurons across experimental groups ( n = 4). Data are expressed as means ± SEM. The difference between the groups was assessed using a one-way ANOVA followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bars: 100 μm (A) , 20 μm (E,F) .

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA) in tris-buffered saline containing 0.1% Tween 20 (TBS-T) at room temperature for 2 h, followed by overnight incubation at 4 °C with primary antibodies: mouse anti-JAK1 antibody (1:1000, 66466-1-Ig, Proteintech, Chicago, IL, USA), rabbit anti-pJAK1 antibody (1:1000, AF2012, Affinity, Jiangsu, China), rabbit anti-STAT3 antibody (1:1000, 10253-2-AP, Proteintech), rabbit anti-pSTAT3 antibody (1:1000, AF3293, Affinity), and mouse anti-GAPDH antibody (1:1000, AF0006, Beyotime).

    Techniques: Immunofluorescence, Staining, Marker, Western Blot, Comparison